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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
10/11/2023 |
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Actualizado : |
10/11/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
BORGES, M.A.; CURCIO, B.R.; GASTAL, G.D.A.; GHENO, L.; JUNIOR, A.S.V.; CORCINI, C.D.; NOGUEIRA, C.E.W.; AGUIAR, F.L.N.; GASTAL, E.L. |
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Afiliación : |
MORGANA A. BORGES, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; BRUNA R. CURCIO, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; GUSTAVO DESIRE ANTUNES GASTAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUIZA GHENO, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; ANTONIO S. VARELA JUNIOR, Department of Comparative Animal Reproduction, Institute of Biological Sciences, Federal University of Rio Grande, RS, Rio Grande, Brazil; CARINE D. CORCINI, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; CARLOS E.W. NOGUEIRA, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; FRANCISCO L.N. AGUIAR, Department of Veterinary Medicine, Sousa Campus, Federal Institute of Education, Science and Technology of Paraiba, PB, Sousa, Brazil; EDUARDO L. GASTAL, Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, United States. |
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Título : |
Ethanol, Carnoy, and paraformaldehyde as fixative solutions for histological evaluation of preantral follicles in equine ovarian tissue. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Reproductive Biology, December 2023, Volume 23, Issue 4, Article 100814. https://doi.org/10.1016/j.repbio.2023.100814 |
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ISSN : |
1642-431X |
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DOI : |
10.1016/j.repbio.2023.100814 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 28 February 2023; Received in revised form 24 September 2023; Accepted 27 September 2023; Available online 25 October 2023. -- Correspondence author: Gastal, E.L.; Animal Science, School of Agricultural Sciences, Southern Illinois University, 1205 Lincoln Drive, MC 4417, Carbondale, IL, United States; email:egastal@siu.edu -- FUNDING: This research was supported by grants from the Brazilian Criollo Horse Breeder Association (ABCCC) and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS; grant no. 21/2551-0002239-6). A.S. Varela Junior, C.D. Corcini, and B.R. Curcio had research productivity fellowships from the National Council for Scientific and Technological Development (CNPq). M.A. Borges and L. Gheno received DS and MS scholarships, respectively, from the Coordination for the Improvement of Higher Education Personnel (CAPES), Brazil. -- |
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Contenido : |
ABSTRACT.- The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more time-consuming and expensive techniques. Additional studies concerning the impact of different fixatives on the ultrastructure of cellular populations and their compatibility with IHC and molecular techniques in equine ovarian tissue are warranted. © 2023 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn MenosABSTRACT.- The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more ... Presentar Todo |
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Palabras claves : |
Fixative solution; Mare; Ovarian tissue quality; Preantral follicle morphometry. |
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Asunto categoría : |
L50 Fisiología y bioquímica animal |
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Marc : |
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001 1064365
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022 $a1642-431X
024 7 $a10.1016/j.repbio.2023.100814$2DOI
100 1 $aBORGES, M.A.
245 $aEthanol, Carnoy, and paraformaldehyde as fixative solutions for histological evaluation of preantral follicles in equine ovarian tissue.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 28 February 2023; Received in revised form 24 September 2023; Accepted 27 September 2023; Available online 25 October 2023. -- Correspondence author: Gastal, E.L.; Animal Science, School of Agricultural Sciences, Southern Illinois University, 1205 Lincoln Drive, MC 4417, Carbondale, IL, United States; email:egastal@siu.edu -- FUNDING: This research was supported by grants from the Brazilian Criollo Horse Breeder Association (ABCCC) and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS; grant no. 21/2551-0002239-6). A.S. Varela Junior, C.D. Corcini, and B.R. Curcio had research productivity fellowships from the National Council for Scientific and Technological Development (CNPq). M.A. Borges and L. Gheno received DS and MS scholarships, respectively, from the Coordination for the Improvement of Higher Education Personnel (CAPES), Brazil. --
520 $aABSTRACT.- The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more time-consuming and expensive techniques. Additional studies concerning the impact of different fixatives on the ultrastructure of cellular populations and their compatibility with IHC and molecular techniques in equine ovarian tissue are warranted. © 2023 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn
653 $aFixative solution
653 $aMare
653 $aOvarian tissue quality
653 $aPreantral follicle morphometry
700 1 $aCURCIO, B.R.
700 1 $aGASTAL, G.D.A.
700 1 $aGHENO, L.
700 1 $aJUNIOR, A.S.V.
700 1 $aCORCINI, C.D.
700 1 $aNOGUEIRA, C.E.W.
700 1 $aAGUIAR, F.L.N.
700 1 $aGASTAL, E.L.
773 $tReproductive Biology, December 2023, Volume 23, Issue 4, Article 100814. https://doi.org/10.1016/j.repbio.2023.100814
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| 66. |  | Abadie, R.Ceretta, S. Exploring crop adaptation through the study of multi environment trials (mets) ln: South American Oats Congress, 3., 1997, La Estanzuela, Colonia, Uruguay Rebuffo, M.; Abadie, T., eds. [Anales]. La Estanzuela, Colonia (Uruguay): INIA, 1997. p. 35-40| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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| 68. | | Ceretta, S. Girasol ln: Jornada Cultivos de Verano, 1991 set 11 : La Estanzuela [Memorias]. Montevideo (Uruguay): INIA, 1991. p19-46 Jornada de Cultivos de Verano (INIA)| Biblioteca(s): INIA La Estanzuela. |
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| 69. | | CERETTA, S.; VILARO, D. Girasol: Evaluación de cultivares In: GIMÉNEZ, A.; RESTAINO, E. (Eds.). Girasol y soja : algunos aspectos tecnológicos de producción para el litoral oeste de Uruguay. Montevideo (Uruguay): INIA, 1994. p79-98 (INIA Boletin de Divulgacion; 47) Convenio INIA - Cámara de Aceites Comestibles - Facultad de Agronomía| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| 71. | | GONZÁLEZ BARRIOS, P.; PÉREZ, O.; CASTRO, M.; CERETTA, S.; VILARO, D.; GUTIÉRREZ, L. Identificación de limitantes a la expresión del potencial de rendimiento en girasol en Uruguay mediante GGE biplots y PLS regression. In: IV Encuentro Iberoamericano de Biometría; 4o. y XVIII Reunión Científica del GAB, 17o., Setiembre 2013, Mar del Plata ,ROMERO, M.C.; MARINELLI, C.; CEPEDA, R. Eds., La Plata, Bs As, Argentina: Grupo Argentino de Biometría. p. 236-239| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA La Estanzuela. |
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| 72. | | DÍAZ DE ACKERMANN, M.; KOHLI, M.M.; CERETTA, S.; ABADIE, T.; IBAÑEZ, W. Importancia de las enfermedades en la producción del trigo en Uruguay. In: EXPLORANDO ALTOS RENDIMIENTOS DE TRIGO, 1997, LA ESTANZUELA, COLONIA, UY. [Taller]. [Montevido, UY]: CIMMYT-INIA, 1997. p. 261-278.| Tipo: Presentaciones Orales |
| Biblioteca(s): INIA La Estanzuela. |
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| 74. | | CERETTA, S. INIA está reiniciando su programa de mejoramiento genético de soja. In: MESA TECNOLÓGICA DE OLEAGINOSOS (MTO), UY. "Aumentar la producción y disminuir impactos". Verde (Montevideo), v.2, n. 11, p. 106, 2009. "La Mesa Tecnológica de Oleaginosos (MTO) se creó hace cuatro años [i.e. 2005] a partir de una iniciativa público-privada que agrupa empresas productores, industriales y comercializadoras de oleaginosos, así como instituciones de...| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA La Estanzuela. |
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| 80. | | LARZÁBAL, J.; YAMANAKA, N.; CERETTA, S.; RODRIGUEZ, M.; STEWART, S. Introgression of Asian soybean rust resistant genes into elite soybean lines from Uruguay. International Journal of Pest Management, 2022, vol. 68, issue 4: "Uruguayan Society of Phytopathology (SUFIT): Plant protection for a sustainable agriculture", p.319-327. doi: https://doi.org/10.1080/09670874.2022.2118894 Article history: Received 03 May 2022, Accepted 23 August 2022, Published online: 11 November 2022. Corresponding author: Silvina Stewart, Instituto Nacional de Investigación Agropecuaria (INIA), Programa Nacional de Cultivos de Secano,...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
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